产品名称
ASAH2 Rabbit Polyclonal Antibody
蛋白名称
Neutral ceramidase (N-CDase) (NCDase) (EC 3.5.1.23) (Acylsphingosine deacylase 2) (BCDase) (LCDase) (hCD) (N-acylsphingosine amidohydrolase 2) (Non-lysosomal ceramidase) [Cleaved into: Neutral ceramidase soluble form]
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56624
Human Swissprot No.
Q9NR71
Human Swissprot Link
https://www.uniprot.org/uniprot/Q9NR71
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54447
Mouse Swissprot No.
Q9JHE3
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9JHE3/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=114104
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q91XT9/entry
特异性
This antibody detects endogenous levels of ASAH2 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Primarily expressed in intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). The ubiquitous expression observed for ASAH2 might be an experimental artifact due to the paralog ASAH2B (PubMed:17334805).
细胞定位
[Neutral ceramidase]: Cell membrane ; Single-pass type II membrane protein . Membrane raft ; Single-pass type II membrane protein . Membrane, caveola ; Single-pass type II membrane protein . Golgi apparatus membrane ; Single-pass type II membrane protein . Mitochondrion . Secreted, extracellular exosome . Enriched in exosomes upon stimulation by cytokine (PubMed:24798654). Enriched in caveolae and lipid rafts (By similarity). The localization to the mitochondrion could not be confirmed (PubMed:15845354). .; [Neutral ceramidase soluble form]: Secreted .
功能
Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH . Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation . Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine . Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells . Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity). This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity).
