产品名称
LRRC8A/SWELL1 Rabbit Polyclonal Antibody
基因名称
LRRC8A KIAA1437 LRRC8 UNQ221/PRO247
蛋白名称
Leucine-rich repeat-containing protein 8A
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56262
Human Swissprot No.
Q8IWT6
Human Swissprot Link
https://www.uniprot.org/uniprot/Q8IWT6
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=241296
Mouse Swissprot No.
Q80WG5
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q80WG5/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=311846
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q4V8I7/entry
特异性
This antibody detects endogenous levels of LRRC8A/SWELL1 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Expressed in brain, kidney, ovary, lung, liver, heart, and fetal brain and liver. Found at high levels in bone marrow; lower levels are detected in peripheral blood cells. Expressed on T-cells as well as on B-lineage cells.
细胞定位
Cell membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Mainly localizes to the cell membrane, with some intracellular localization to lysosomes. .
功能
Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes . The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine . Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress . LRRC8A and LRRC8D are required for the uptake of the drug cisplatin . In complex with LRRC8C or LRRC8E, acts as a transporter of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol: mediates both import and export of 2'-3'-cGAMP, thereby promoting transfer of 2'-3'-cGAMP to bystander cells . In contrast, complexes containing LRRC8D inhibit transport of 2'-3'-cGAMP . Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition . Can form functional channels by itself (in vitro) . Involved in B-cell development: required for the pro-B cell to pre-B cell transition . Also required for T-cell development (By similarity). Required for myoblast differentiation: VRAC activity promotes membrane hyperpolarization and regulates insulin-stimulated glucose metabolism and oxygen consumption (By similarity). Also acts as a regulator of glucose-sensing in pancreatic beta cells: VRAC currents, generated in response to hypotonicity- or glucose-induced beta cell swelling, depolarize cells, thereby causing electrical excitation, leading to increase glucose sensitivity and insulin secretion . Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition: lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis .
