产品名称
NLK Rabbit Polyclonal Antibody
蛋白名称
Serine/threonine-protein kinase NLK (EC 2.7.11.24) (Nemo-like kinase) (Protein LAK1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9UBE8
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9UBE8/entry
Mouse Swissprot No.
O54949
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O54949
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941D3ZSZ3
免疫原
Synthesized peptide derived from human protein . at AA range: 230-310
特异性
NLK Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by tyrosine and threonine phosphorylation (By similarity). Activated by activin.,function:Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.,PTM:Dually phosphorylated on Thr-291 and Tyr-293, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Predominantly nuclear. A smaller fraction is cytoplasmic.,subunit:Interacts with STAT3 (By similarity). Interacts with RNF138/NARF and TCF7L2/TCF4. Interacts with HIPK2 and MYB.,
组织表达
Amygdala,Placenta,T-cell,Uterus,
细胞定位
Nucleus . Cytoplasm . Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity). .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;WNT;WNT-T CELLAdherens_Junction;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by tyrosine and threonine phosphorylation (By similarity). Activated by activin.,function:Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.,PTM:Dually phosphorylated on Thr-291 and Tyr-293, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Predominantly nuclear. A smaller fraction is cytoplasmic.,subunit:Interacts with STAT3 (By similarity). Interacts with RNF138/NARF and TCF7L2/TCF4. Interacts with HIPK2 and MYB.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
