产品名称
VPS4A Rabbit Polyclonal Antibody
蛋白名称
Vacuolar protein sorting-associated protein 4A (Protein SKD2) (VPS4-1) (hVPS4)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=27183
Human Swissprot No.
Q9UN37
Human Swissprot Link
https://www.uniprot.org/uniprot/Q9UN37
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116733
Mouse Swissprot No.
Q8VEJ9
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8VEJ9/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=246772
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q793F9/entry
特异性
This antibody detects endogenous levels of VPS4A at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Ubiquitously expressed.
细胞定位
Late endosome membrane ; Peripheral membrane protein . Midbody . Cytoplasm, cytoskeleton, spindle . Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody (PubMed:24814515). Localized in two distinct rings on either side of the Flemming body. .
功能
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. It is required for proper accomplishment of various processes including the regulation of endosome size, primary cilium organization, mitotic spindle organization, chromosome segregation, and nuclear envelope sealing and spindle disassembly during anaphase . Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission . VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan . Critical for normal erythroblast cytokinesis and correct erythropoiesis . ; (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
