产品名称
ARHG4 Rabbit Polyclonal Antibody
蛋白名称
Rho guanine nucleotide exchange factor 4 (APC-stimulated guanine nucleotide exchange factor 1) (Asef) (Asef1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9NR80
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9NR80/entry
Mouse Swissprot No.
Q7TNR9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q7TNR9
免疫原
Synthesized peptide derived from human protein . at AA range: 270-350
特异性
ARHG4 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013],
组织表达
Expressed at high levels in the brain, skeletal muscle and testis and at low levels in the kidney, lung, small intestine, ovary and prostate. Expression is aberrantly enhanced in most colorectal tumors.
细胞定位
[Isoform 3]: Cytoplasm. Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Associated with membrane ruffles. .
信号通路
Regulates Actin and Cytoskeleton;
功能
function:Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.,sequence caution:Translation N-terminally extended.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH3 domain.,subcellular location:Associated with membrane ruffles.,subunit:Isoform 3 interacts with RHOA and RAC1, and through its N-terminus with APC. Found in a complex consisting of ARHGEF4, APC and CTNNB1.,tissue specificity:Expressed at low levels in brain, kidney, lung and muscle.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
