产品名称
RHG24 Rabbit Polyclonal Antibody
蛋白名称
Rho GTPase-activating protein 24 (Filamin-A-associated RhoGAP) (FilGAP) (RAC1- and CDC42-specific GTPase-activating protein of 72 kDa) (RC-GAP72) (Rho-type GTPase-activating protein 24) (RhoGAP of 73 kDa) (Sarcoma antigen NY-SAR-88) (p73RhoGAP)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q8N264
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8N264/entry
Mouse Swissprot No.
Q8C4V1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8C4V1
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q5U2Z7
免疫原
Synthesized peptide derived from human protein . at AA range: 550-630
特异性
RHG24 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a Rho-GTPase activating protein, which is specific for the small GTPase family member Rac. Binding of the encoded protein by filamin A targets it to sites of membrane protrusion, where it antognizes Rac. This results in suppression of lamellae formation and promotion of retraction to regulate cell polarity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016],
组织表达
Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells.
细胞定位
Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction, focal adhesion. Cell projection. Localizes to actin stress fibers. In migrating cells, localizes to membrane lamellae and protusions.
功能
domain:The coiled coil domain mediates the interaction with FLNA leading to its recruitment to lamellae.,function:Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.,induction:Isoform 2 is up-regulated during capillary tube formation in umbilical vein endothelial cells.,PTM:Phosphorylated by ROCK, leading to activate the RacGAP activity.,similarity:Contains 1 PH domain.,similarity:Contains 1 Rho-GAP domain.,subcellular location:Localizes to actin stress fibers. In migrating cells, localizes to membrane lamellae and protusions.,subunit:Interacts with FLNA.,tissue specificity:Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
