产品名称
Intersectin 1 Rabbit Polyclonal Antibody
蛋白名称
Intersectin-1 (SH3 domain-containing protein 1A) (SH3P17)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6453
Human Swissprot No.
Q15811
Human Swissprot Link
https://www.uniprot.org/uniprot/Q15811
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16443
Mouse Swissprot No.
Q9Z0R4
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9Z0R4/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29491
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q9WVE9/entry
特异性
This antibody detects endogenous levels of Intersectin 1 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Isoform 1 is expressed almost exclusively in the brain. Isoform 2 is detected in brain, spleen, lung, liver, heart, skeletal muscle and kidney. Isoform 5 is primarily expressed in brain, spleen, lung and kidney (at protein level) (PubMed:21712076). Isoform 1 and isoform 2 are detected in brain (PubMed:10482960). Isoform 2 is ubiquitous in adult and fetal tissues with high expression in skeletal muscle, heart, spleen, ovary, testis and all fetal tissues tested and low expression in thymus, blood, lung, liver and pancreas. Isoform 1 is expressed almost exclusively in the brain, in all brain regions. Not expressed in the spinal cord (PubMed:9799604, PubMed:21712076, PubMed:11690630).
细胞定位
Endomembrane system . Cell junction, synapse, synaptosome . Cell projection, lamellipodium . Cell membrane . Membrane, clathrin-coated pit . Recycling endosome . Endosome . Cytoplasmic vesicle . Colocalizes with SGIP1 at the plasma membrane in structures corresponding most probably to clathrin-coated pits (PubMed:20946875). Colocalizes with RAB13 on cytoplasmic vesicles that are most likely recycling endosomes (PubMed:29030480). .; [Isoform 2]: Cytoplasm . Endomembrane system . Nucleus envelope . Shuttles between the cytoplasm and nucleus in an XPO1/CRM1-dependent manner. .; [Isoform 5]: Endomembrane system .
功能
Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery . Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex . Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits . Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (By similarity). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 . Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (By similarity). Inhibits ARHGAP31 activity toward RAC1 . ; [Isoform 1]: Plays a role in synaptic vesicle endocytosis in brain neurons.
