产品名称
CSRP3 Rabbit Polyclonal Antibody
蛋白名称
Cysteine and glycine-rich protein 3 (Cardiac LIM protein) (Cysteine-rich protein 3) (CRP3) (LIM domain protein, cardiac) (Muscle LIM protein)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8048
Human Swissprot No.
P50461
Human Swissprot Link
https://www.uniprot.org/uniprot/P50461
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13009
Mouse Swissprot No.
P50462
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P50462/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=117505
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P50463/entry
特异性
This antibody detects endogenous levels of CSRP3 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Cardiac and slow-twitch skeletal muscles. Isoform 2 is expressed in striated muscle. Isoform 2 is specifically expressed at higher levels in patients with neuromuscular diseases, such as limb-girdle muscular dystrophy 2A (LGMD2A), Duchenne muscular dystrophy (DMD) and dermatomyositis (PubMed:24860983).
细胞定位
Nucleus . Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm, myofibril, sarcomere . Nucleocytoplasmic shuttling protein. Mainly cytoplasmic. In the Z line, found associated with GLRX3 (By similarity). .; [Isoform 2]: Cytoplasm, myofibril, sarcomere, Z line .
功能
Positive regulator of myogenesis. Acts as cofactor for myogenic bHLH transcription factors such as MYOD1, and probably MYOG and MYF6. Enhances the DNA-binding activity of the MYOD1:TCF3 isoform E47 complex and may promote formation of a functional MYOD1:TCF3 isoform E47:MEF2A complex involved in myogenesis (By similarity). Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation (By similarity). The role in regulation of cytoskeleton dynamics by association with CFL2 is reported conflictingly: Shown to enhance CFL2-mediated F-actin depolymerization dependent on the CSRP3:CFL2 molecular ratio, and also shown to reduce the ability of CLF1 and CFL2 to enhance actin depolymerization . Proposed to contribute to the maintenance of muscle cell integrity through an actin-based mechanism. Can directly bind to actin filaments, cross-link actin filaments into bundles without polarity selectivity and protect them from dilution- and cofilin-mediated depolymerization; the function seems to involve its self-association . In vitro can inhibit PKC/PRKCA activity . Proposed to be involved in cardiac stress signaling by down-regulating excessive PKC/PRKCA signaling (By similarity). ; [Isoform 2]: May play a role in early sarcomere organization. Overexpression in myotubes negatively regulates myotube differentiation. By association with isoform 1 and thus changing the CSRP3 isoform 1:CFL2 stoichiometry is proposed to down-regulate CFL2-mediated F-actin depolymerization.
