产品名称
PAF1 Rabbit Polyclonal Antibody
蛋白名称
RNA polymerase II-associated factor 1 homolog (hPAF1) (Pancreatic differentiation protein 2)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54623
Human Swissprot No.
Q8N7H5
Human Swissprot Link
https://www.uniprot.org/uniprot/Q8N7H5
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54624
Mouse Swissprot No.
Q8K2T8
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8K2T8/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=361531
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q4V886/entry
特异性
This antibody detects endogenous levels of PAF1 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Nucleus . Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin.
功能
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.
