产品名称
IA-2/PTPRN Rabbit Polyclonal Antibody
蛋白名称
Receptor-type tyrosine-protein phosphatase-like N (R-PTP-N) (Islet cell antigen 512) (ICA 512) (Islet cell autoantigen 3) (PTP IA-2)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5798
Human Swissprot No.
Q16849
Human Swissprot Link
https://www.uniprot.org/uniprot/Q16849
Mouse Swissprot No.
Q60673
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q60673/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116660
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q63259/entry
特异性
This antibody detects endogenous levels of IA-2/PTPRN at Human,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Expression is restricted to neuroendocrine cells. Found in pancreas, brain and pituitary.
细胞定位
Membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type I membrane protein . Perikaryon . Cell projection, axon . Cell junction, synapse . Cell membrane ; Single-pass type I membrane protein . Endosome . Detected on neuronal secretory vesicles, but not on synaptic vesicles. Colocalizes with insulin-containing secretory granules (PubMed:25561468). Primarily detected on secretory vesicle membranes. Transiently found at the cell membrane, when secretory vesicles fuse with the cell membrane to release their cargo. Is then endocytosed and recycled to secretory vesicles via the Golgi apparatus membranes. .; [ICA512-transmembrane fragment]: Cytoplasmic vesicle, secretory vesicle membrane .; [ICA512-cleaved cytosolic fragment]: Nucleus .
功能
Plays a role in vesicle-mediated secretory processes . Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets (By similarity). Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation . Plays a role in insulin secretion in response to glucose stimuli . Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain (By similarity). In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (By similarity). Required to maintain normal levels of renin expression and renin release (By similarity). Seems to lack intrinsic enzyme activity (By similarity). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (By similarity). ; [ICA512-transmembrane fragment]: ICA512-TMF regulates dynamics and exocytosis of insulin secretory granules (SGs); binding of ICA512-TMF to SNTB2/beta-2-syntrophin is proposed to restrain SGs mobility and exocytosis by tethering them to the actin cytoskeleton depending on UTRN; the function is inhibited by cytoplasmic ICA512-CFF dimerizing with ICA512-TMF and displacing SNTB2. ; [ICA512-cleaved cytosolic fragment]: ICA512-CCF translocated to the nucleus promotes expression of insulin and other granule-related genes; the function implicates binding to and regulating activity of STAT5B probably by preventing its dephosphorylation and potentially by inducing its sumoylation by recruiting PIAS4 . Enhances pancreatic beta-cell proliferation by converging with signaling by STAT5B and STAT3 . ICA512-CCF located in the cytoplasm regulates dynamics and exocytosis of insulin secretory granules (SGs) by dimerizing with ICA512-TMF and displacing SNTB2 thus enhancing SGs mobility and exocytosis .
