产品名称
FBXO5 Rabbit Polyclonal Antibody
蛋白名称
F-box only protein 5 (Early mitotic inhibitor 1)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26271
Human Swissprot No.
Q9UKT4
Human Swissprot Link
https://www.uniprot.org/uniprot/Q9UKT4
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=67141
Mouse Swissprot No.
Q7TSG3
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q7TSG3/entry
特异性
This antibody detects endogenous levels of FBXO5 at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration (PubMed:11988738). In mitotic cells, localizes throughout the cell, particularly at the spindle (PubMed:15469984). .
功能
Regulator of APC activity during mitotic and meiotic cell cycle . During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex . During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase . Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment . As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities . Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence . During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity). Promotes migration and osteogenic differentiation of mesenchymal stem cells .
