产品名称
CRACR2A Rabbit Polyclonal Antibody
蛋白名称
EF-hand calcium-binding domain-containing protein 4B (Calcium release-activated calcium channel regulator 2A) (CRAC channel regulator 2A)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84766
Human Swissprot No.
Q9BSW2
Human Swissprot Link
https://www.uniprot.org/uniprot/Q9BSW2
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=381812
Mouse Swissprot No.
Q3UP38
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q3UP38/entry
特异性
This antibody detects endogenous levels of CRACR2A at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
[Isoform 1]: Expressed in the Jurkat T-cell line. ; [Isoform 2]: Expressed in endothelial cells (PubMed:25475730). Expressed in Weibel-Palade bodies (which are P-selectin/SELP negative) in endothelial cells (PubMed:31092558). Expressed in the Jurkat T-cell line (PubMed:30814157, PubMed:27016526).
细胞定位
[Isoform 1]: Cytoplasm .; [Isoform 2]: Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center . Cell membrane . Golgi apparatus membrane . Golgi apparatus, trans-Golgi network membrane . Vesicle . T-cell activation-induced elevation of intracellular of Ca(2+) stimulates its transport toward the microtubule organizing center (MTOC) (PubMed:30814157). Histamine stimulation induces a trafficking to the MTOC in a GTP-binding-dependent but Ca(2+)-binding-independent manner (PubMed:31092558). Localizes to Golgi membrane in resting T-cells and upon its interaction with VAV1, is translocated from the Golgi membrane to the immunological synapse via subsynaptic vesicles (PubMed:27016526). Its localization in the Golgi membrane requires isoprenylation and GTP-binding (PubMed:27016526). .
功能
[Isoform 1]: Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex. ; [Isoform 2]: Rab GTPase that mediates the trafficking of Weibel-Palade bodies (WPBs) to microtubule organizing center (MTOC) in endothelial cells in response to acute inflammatory stimuli . During histamine (but not thrombin) stimulation of endothelial cells, the dynein-bound form induces retrograde transport of a subset of WPBs along microtubules to the MTOC in a Ca(2+)-independent manner and its GTPase activity is essential for this function . Ca(2+)-regulated dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules and regulates endosomal trafficking of CD47 . Acts as an intracellular signaling module bridging two important T-cell receptor (TCR) signaling pathways, Ca(2+)-NFAT and JNK, to affect T-cell activation . In resting T-cells, is predominantly localized near TGN network in a GTP-bound form, upon TCR stimulation, localizes at the immunological synapse via interaction with VAV1 to activate downstream Ca(2+)-NFAT and JNK signaling pathways . Plays a role in T-helper 1 (Th1) cell differentiation and T-helper 17 (Th17) cell effector function . Plays a role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation .
