产品名称
RPGF4 Rabbit Polyclonal Antibody
蛋白名称
Rap guanine nucleotide exchange factor 4 (Exchange factor directly activated by cAMP 2) (Exchange protein directly activated by cAMP 2) (EPAC 2) (cAMP-regulated guanine nucleotide exchange factor II) (cAMP-GEFII)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q8WZA2
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8WZA2/entry
Mouse Swissprot No.
Q9EQZ6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9EQZ6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q9Z1C7
免疫原
Synthesized peptide derived from human protein . at AA range: 240-320
特异性
RPGF4 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
domain:The DEP domain is involved in membrane localization independent from regulation by cAMP.,domain:The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.,function:Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.,similarity:Contains 1 DEP domain.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,similarity:Contains 2 cyclic nucleotide-binding domains.,subunit:Interacts with RIMS1 and RIMS2. Probably part of a complex with RIMS2 and GTP-activated RAB3A.,tissue specificity:Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.,
组织表达
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
细胞定位
Cytoplasm. Membrane ; Peripheral membrane protein .
信号通路
Leukocyte transendothelial migration;
功能
domain:The DEP domain is involved in membrane localization independent from regulation by cAMP.,domain:The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2.,function:Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.,similarity:Contains 1 DEP domain.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,similarity:Contains 2 cyclic nucleotide-binding domains.,subunit:Interacts with RIMS1 and RIMS2. Probably part of a complex with RIMS2 and GTP-activated RAB3A.,tissue specificity:Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
