产品名称
INSIG1 Rabbit Polyclonal Antibody
蛋白名称
Insulin-induced gene 1 protein (INSIG-1)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3638
Human Swissprot No.
O15503
Human Swissprot Link
https://www.uniprot.org/uniprot/O15503
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=231070
Mouse Swissprot No.
Q8BGI3
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8BGI3/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64194
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q08755/entry
特异性
This antibody detects endogenous levels of INSIG1 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Expressed in all tissues tested with highest expression in the liver.
细胞定位
Endoplasmic reticulum membrane ; Multi-pass membrane protein .
功能
Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR . Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 . Binds oxysterol, including 25-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum . In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi . Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG1 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2 . Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligases AMFR/gp78 and/or RNF139 . Also regulates degradation of SOAT2/ACAT2 when the lipid levels are low: initiates the ubiquitin-mediated degradation of SOAT2/ACAT2 via recruitment of the ubiquitin ligases AMFR/gp78 .
