产品名称
RNF20 Rabbit Polyclonal Antibody
蛋白名称
E3 ubiquitin-protein ligase BRE1A (BRE1-A) (hBRE1) (EC 6.3.2.-) (RING finger protein 20)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56254
Human Swissprot No.
Q5VTR2
Human Swissprot Link
https://www.uniprot.org/uniprot/Q5VTR2
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=109331
Mouse Swissprot No.
Q5DTM8
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q5DTM8/entry
特异性
This antibody detects endogenous levels of RNF20 at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Expressed in the normal brain and also in malignant gliomas (at protein level).
功能
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
