产品名称
FBXW7 Rabbit Polyclonal Antibody
基因名称
FBXW7 FBW7 FBX30 SEL10
蛋白名称
F-box/WD repeat-containing protein 7 (Archipelago homolog) (hAgo) (F-box and WD-40 domain-containing protein 7) (F-box protein FBX30) (SEL-10) (hCdc4)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55294
Human Swissprot No.
Q969H0
Human Swissprot Link
https://www.uniprot.org/uniprot/Q969H0
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=50754
Mouse Swissprot No.
Q8VBV4
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8VBV4/entry
特异性
This antibody detects endogenous levels of FBXW7 at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
[Isoform 1]: Widely expressed. ; [Isoform 3]: Expressed in brain.
细胞定位
[Isoform 1]: Nucleus, nucleoplasm . Chromosome . Localizes to site of double-strand breaks following phosphorylation by ATM. .; [Isoform 2]: Cytoplasm .; [Isoform 3]: Nucleus, nucleolus .
功能
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins . Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination . Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, RICTOR, and probably PSEN1 . Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation . Involved in bone homeostasis and negative regulation of osteoclast differentiation . Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination . Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage . The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining .
