产品名称
NEU4 Rabbit Polyclonal Antibody
蛋白名称
Sialidase-4 (EC 3.2.1.18) (N-acetyl-alpha-neuraminidase 4)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=129807
Human Swissprot No.
Q8WWR8
Human Swissprot Link
https://www.uniprot.org/uniprot/Q8WWR8
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=241159
Mouse Swissprot No.
Q8BZL1
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8BZL1/entry
特异性
This antibody detects endogenous levels of NEU4 at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
[Isoform 1]: Predominant form in liver. Also expressed in brain, kidney and colon. ; [Isoform 2]: Highly expressed in brain and at lower levels in kidney and liver.
细胞定位
[Isoform 1]: Cell membrane ; Peripheral membrane protein. Endoplasmic reticulum membrane ; Peripheral membrane protein. Microsome membrane ; Peripheral membrane protein. Mitochondrion membrane ; Peripheral membrane protein. Cell projection, neuron projection . Predominantly associates with endoplasmic reticulum membranes. Only a small fraction associates with mitochondrial and plasma membranes. .; [Isoform 2]: Mitochondrion inner membrane ; Peripheral membrane protein. Mitochondrion outer membrane ; Peripheral membrane protein. Lysosome lumen . According to PubMed:15213228, isoform 2 is soluble, N-glycosylated and found in the lumen of lysosomes. However, no signal sequence nor N-glycosylation site is predicted from the sequence.
功能
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Efficiently hydrolyzes gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 . Hydrolyzes poly-alpha-(2->8)-sialylated neural cell adhesion molecule NCAM1 likely at growth cones, suppressing neurite outgrowth in hippocampal neurons (By similarity). May desialylate sialyl Lewis A and X antigens at the cell surface, down-regulating these glycan epitopes recognized by SELE/E selectin in the initiation of cell adhesion and extravasation . Has sialidase activity toward mucin, fetuin and sialyllactose .
