产品名称
ASAH1 Rabbit Polyclonal Antibody
基因名称
ASAH1 ASAH HSD-33 HSD33
蛋白名称
Acid ceramidase (AC) (ACDase) (Acid CDase) (EC 3.5.1.23) (Acylsphingosine deacylase) (N-acylsphingosine amidohydrolase) (Putative 32 kDa heart protein) (PHP32) [Cleaved into: Acid ceramidase subunit alpha; Acid ceramidase subunit beta]
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=427
Human Swissprot No.
Q13510
Human Swissprot Link
https://www.uniprot.org/uniprot/Q13510
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11886
Mouse Swissprot No.
Q9WV54
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9WV54/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84431
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q6P7S1/entry
特异性
This antibody detects endogenous levels of ASAH1 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Broadly expressed with higher expression in heart.
细胞定位
Lysosome . Secreted . Secretion is extremely low and localization to lysosomes is mannose-6-phosphate receptor-dependent. .; [Isoform 2]: Nucleus . Cytoplasm . A localization to the nucleus and the cytoplasm has also been reported for ASAH1, most probably for isoforms devoid of a signal peptide. .
功能
Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH . Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation . Has a higher catalytic efficiency towards C12-ceramides versus other ceramides . Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine . For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used . Has also an N-acylethanolamine hydrolase activity . By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes . Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity). By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis . ; [Isoform 2]: May directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity.
