产品名称
mDia1 Rabbit Polyclonal Antibody
蛋白名称
Protein diaphanous homolog 1 (Diaphanous-related formin-1) (DRF1)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1729
Human Swissprot No.
O60610
Human Swissprot Link
https://www.uniprot.org/uniprot/O60610
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13367
Mouse Swissprot No.
O08808
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/O08808/entry
特异性
This antibody detects endogenous levels of mDia1 at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets (PubMed:26912466).
细胞定位
Cell membrane . Cell projection, ruffle membrane . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm . Nucleus . Membrane ruffles, especially at the tip of ruffles, of motile cells. .
功能
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (By similarity). Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (By similarity). Required for cytokinesis, and transcriptional activation of the serum response factor (By similarity). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells . The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex . It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity . In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization . Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape . Plays a role in brain development . Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (By similarity).
