产品名称
OAS1 Rabbit Polyclonal Antibody
蛋白名称
2'-5'-oligoadenylate synthase 1 ((2-5')oligo(A) synthase 1) (2-5A synthase 1) (EC 2.7.7.-) (E18/E16) (p46/p42 OAS)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4938
Human Swissprot No.
P00973
Human Swissprot Link
https://www.uniprot.org/uniprot/P00973
特异性
This antibody detects endogenous levels of OAS1 at Human
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Cytoplasm . Mitochondrion . Nucleus . Microsome . Endoplasmic reticulum . Secreted . Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions. .; [Isoform p46]: (Microbial infection) In SARS coronavirus-2/SARS-CoV-2 infected cells, prenylated form localizes to membranous perinuclear structures reminiscent of the endoplasmic reticulum rich in viral dsRNA which are SARS-CoV-2 replicative organelles. .; [Isoform p42]: (Microbial infection) In SARS coronavirus-2/SARS-CoV-2 infected cells, since its not prenylated, is diffusely localized and unable to initiate a detectable block to SARS-CoV-2 replication. .
功能
Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response . In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication . Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L. ; [Isoform p46]: When prenylated at C-terminal, acts as a double-stranded RNA (dsRNA) sensor specifically targeted to membranous replicative organelles in SARS coronavirus-2/SARS-CoV-2 infected cells where it binds to dsRNA structures in the SARS-CoV-2 5'-UTR and initiates a potent block to SARS-CoV-2 replication. Recognizes short stretches of dsRNA and activates RNase L. The binding is remarkably specific, with two conserved stem loops in the SARS-CoV-2 5'- untranslated region (UTR) constituting the principal viral target . The same mechanism is necessary to initiate a block to cardiovirus EMCV . ; [Isoform p42]: Not prenylated at C-terminal, is diffusely localized and unable to initiate a detectable block to SARS-CoV-2 replication.
