产品名称
ECHS1 Rabbit Polyclonal Antibody
蛋白名称
Enoyl-CoA hydratase, mitochondrial (EC 4.2.1.17) (Enoyl-CoA hydratase 1) (Short-chain enoyl-CoA hydratase) (SCEH)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1892
Human Swissprot No.
P30084
Human Swissprot Link
https://www.uniprot.org/uniprot/P30084
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=93747
Mouse Swissprot No.
Q8BH95
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8BH95/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100911186
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P14604/entry
特异性
This antibody detects endogenous levels of ECHS1 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Liver, fibroblast, muscle. Barely detectable in spleen and kidney.
细胞定位
Mitochondrion matrix.
功能
Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond . Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 . Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA) . Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate . Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation . At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA) (By similarity).
