产品名称
ASF/SF2 Rabbit Polyclonal Antibody
基因名称
SRSF1 ASF SF2 SF2P33 SFRS1 OK/SW-cl.3
蛋白名称
Serine/arginine-rich splicing factor 1 (Alternative-splicing factor 1) (ASF-1) (Splicing factor, arginine/serine-rich 1) (pre-mRNA-splicing factor SF2, P33 subunit)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6426
Human Swissprot No.
Q07955
Human Swissprot Link
https://www.uniprot.org/uniprot/Q07955
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=110809
Mouse Swissprot No.
Q6PDM2
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q6PDM2/entry
特异性
This antibody detects endogenous levels of ASF/SF2 at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Cytoplasm . Nucleus speckle . In nuclear speckles. Shuttles between the nucleus and the cytoplasm (PubMed:12215544, PubMed:20308322, PubMed:9420331, PubMed:24449914). Nuclear import is mediated via interaction with TNPO3 (PubMed:24449914). .
功能
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
