BD-PN1096

CLP1 Rabbit Polyclonal Antibody

常规抗体

CLP1 HEAB

Polyribonucleotide 5'-hydroxyl-kinase Clp1 (EC 2.7.1.78) (Polynucleotide kinase Clp1) (Pre-mRNA cleavage complex II protein Clp1)

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.

10978

Q92989

https://www.uniprot.org/uniprotkb/Q92989/entry

Q99LI9

http://www.uniprot.org/uniprot/Q99LI9

Q5PQL4

http://www.uniprot.org/uniprot/O54941Q5PQL4

Synthesized peptide derived from human protein . at AA range: 50-130

CLP1 Polyclonal Antibody detects endogenous levels of protein.

WB 1:500-2000 ELISA 1:5000-20000

46kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014],

Lung,Placenta,

Nucleus .

catalytic activity:ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.,catalytic activity:ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.,cofactor:Magnesium, manganese or nickel.,function:Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by dicer1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.,similarity:Belongs to the Clp1 family.,subunit:Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.