产品名称
ISCU Rabbit Polyclonal Antibody
蛋白名称
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (NifU-like N-terminal domain-containing protein) (NifU-like protein)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23479
Human Swissprot No.
Q9H1K1
Human Swissprot Link
https://www.uniprot.org/uniprot/Q9H1K1
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=66383
Mouse Swissprot No.
Q9D7P6
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9D7P6/entry
特异性
This antibody detects endogenous levels of ISCU at Human, Mouse
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta.
细胞定位
[Isoform 1]: Mitochondrion .; [Isoform 2]: Cytoplasm. Nucleus .
功能
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins . First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity). ; [Isoform 2]: Functions as a cytoplasmic scaffold protein for the de novo synthesis of iron-sulfur clusters in the cytoplasm.
