产品名称
HMGB1 (PTR2339) Mouse Monoclonal Antibody
别名
High mobility group protein B1 (High mobility group protein 1) (HMG-1)
蛋白名称
High mobility group protein B1 (High mobility group protein 1) (HMG-1)
存储缓冲液
PBS, pH7.4, 50% glycerol, 0.03%Proclin 300
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3146
Human Swissprot No.
P09429
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P09429/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100862258
Mouse Swissprot No.
P63158
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P63158/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25459
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P63159/entry
特异性
This antibody detects endogenous levels of HMGB1 at Human, Mouse,Rat
稀释度
WB 1:500-2000 ELISA 1:5000-20000
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Ubiquitous. Expressed in platelets (PubMed:11154118).
细胞定位
Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed:12231511, PubMed:14532127, PubMed:15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed:11154118). An increased chromatin association is observed when associated with the adenovirus protein pVII (PubMed:27362237). .
功能
function:Binds preferentially single-stranded DNA and unwinds double stranded DNA.,similarity:Belongs to the HMGB family.,similarity:Contains 2 HMG box DNA-binding domains.,
