产品名称
Keap1 (PTR2556) Mouse Monoclonal Antibody
别名
Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2) (Kelch-like protein 19)
基因名称
KEAP1 INRF2 KIAA0132 KLHL19
蛋白名称
Kelch-like ECH-associated protein 1 (Cytosolic inhibitor of Nrf2) (INrf2) (Kelch-like protein 19)
反应种属
Human, Mouse, Dog (predicted: Rat)
存储缓冲液
PBS, pH7.4, 50% glycerol, 0.03%Proclin 300
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9817
Human Swissprot No.
Q14145
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q14145/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=50868
Mouse Swissprot No.
Q9Z2X8
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9Z2X8/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=117519
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P57790/entry
特异性
This antibody detects endogenous levels of Keap1 at Human, Mouse,Rat
稀释度
WB 1:500-2000 ELISA 1:5000-20000
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Broadly expressed, with highest levels in skeletal muscle.
细胞定位
Cytoplasm . Nucleus . Mainly cytoplasmic (PubMed:15601839). In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62 (PubMed:20452972). .
功能
disease:Defects in KEAP1 may be a cause of breast cancer.,disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degradation.,similarity:Contains 1 BACK (BTB/Kelch associated) domain.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subcellular location:Shuttles between cytoplasm and nucleus.,subunit:Homodimer. Interacts with the N-terminal regulatory domain of NF2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Interacts with PGAM5.,tissue specificity:Broadly expressed, with highest levels in skeletal muscle.,
