产品名称
LIMK-1/2 (PTR2545) Mouse Monoclonal Antibody
别名
LIM domain kinase 1 (LIMK-1) (EC 2.7.11.1)
蛋白名称
LIM domain kinase 1 (LIMK-1) (EC 2.7.11.1)
反应种属
Human, Mouse (predicted: Rat)
存储缓冲液
PBS, pH7.4, 50% glycerol, 0.03%Proclin 300
Human Swissprot No.
P53667/P53671
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P53667/entry
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P53669/entry
特异性
This antibody detects endogenous levels of LIMK-1/2 at Human, Mouse,Rat
稀释度
WB 1:500-2000 ELISA 1:5000-20000
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
细胞定位
Cytoplasm . Nucleus . Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Predominantly found in the cytoplasm. Localizes in the lamellipodium in a CDC42BPA, CDC42BPB and FAM89B/LRAP25-dependent manner. .
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,function:Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.,PTM:Autophosphorylated.,PTM:Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 LIM zinc-binding domains.,subunit:Self-associates. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with SSH1. Interacts with NISCH.,tissue specificity:Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.,
