Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:
16916645, PubMed:
17938197, PubMed:
19111657, PubMed:
19589784, PubMed:
28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed:
16916645, PubMed:
17938197, PubMed:
19111657, PubMed:
19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed:
19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:
20061386).
