产品名称
PABP4 Rabbit Polyclonal Antibody
蛋白名称
Polyadenylate-binding protein 4 (PABP-4) (Poly(A)-binding protein 4) (Activated-platelet protein 1) (APP-1) (Inducible poly(A)-binding protein) (iPABP)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q13310
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q13310/entry
免疫原
Synthesized peptide derived from human protein . at AA range: 190-270
特异性
PABP4 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript va
组织表达
Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.
细胞定位
Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
功能
function:Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo.,PTM:Arg-518 is dimethylated, probably to asymmetric dimethylarginine.,similarity:Belongs to the polyadenylate-binding protein type-1 family.,similarity:Contains 1 PABC domain.,similarity:Contains 4 RRM (RNA recognition motif) domains.,subunit:Interacts with NFX1.,tissue specificity:Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
