产品名称
P4K2A Rabbit Polyclonal Antibody
蛋白名称
Phosphatidylinositol 4-kinase type 2-alpha (EC 2.7.1.67) (Phosphatidylinositol 4-kinase type II-alpha)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9BTU6
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9BTU6/entry
Mouse Swissprot No.
Q2TBE6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q2TBE6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q99M64
免疫原
Synthesized peptide derived from human protein . at AA range: 50-130
特异性
P4K2A Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008],
组织表达
Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.
细胞定位
Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor . Membrane raft . Cell projection, dendrite . Cell junction, synapse, presynaptic cell membrane . Cell junction, synapse, synaptosome . Mitochondrion . Endosome . Cytoplasmic vesicle . Membrane ; Lipid-anchor . Cell membrane . Perikaryon . Cell projection, neuron projection . Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. Transported from neuronal cell body to neuron projections and neurite tips in a BLOC-1- and AP-3-complexes-dependent manner. .
功能
catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.,function:Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells.,similarity:Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.,similarity:Contains 1 PI3K/PI4K domain.,subcellular location:Found in subdomains of the plasma membrane termed non-caveolar membrane rafts.,tissue specificity:Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
