产品名称
P4K2A Rabbit Polyclonal Antibody
蛋白名称
Phosphatidylinositol 4-kinase type 2-alpha (EC 2.7.1.67) (Phosphatidylinositol 4-kinase type II-alpha)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9BTU6
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9BTU6/entry
Mouse Swissprot No.
Q2TBE6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q2TBE6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q99M64
免疫原
Synthesized peptide derived from human protein . at AA range: 50-130
特异性
P4K2A Polyclonal Antibody detects endogenous levels of protein.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008],
组织表达
Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.
细胞定位
Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor . Membrane raft . Cell projection, dendrite . Cell junction, synapse, presynaptic cell membrane . Cell junction, synapse, synaptosome . Mitochondrion . Endosome . Cytoplasmic vesicle . Membrane ; Lipid-anchor . Cell membrane . Perikaryon . Cell projection, neuron projection . Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. Transported from neuronal cell body to neuron projections and neurite tips in a BLOC-1- and AP-3-complexes-dependent manner. .
功能
catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.,function:Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells.,similarity:Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.,similarity:Contains 1 PI3K/PI4K domain.,subcellular location:Found in subdomains of the plasma membrane termed non-caveolar membrane rafts.,tissue specificity:Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
