产品名称
PSPC1 Rabbit Polyclonal Antibody
蛋白名称
Paraspeckle component 1 (Paraspeckle protein 1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q8WXF1
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8WXF1/entry
Mouse Swissprot No.
Q8R326
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8R326
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q4KLH4
免疫原
Synthesized peptide derived from human protein . at AA range: 10-90
特异性
PSPC1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles, known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA, NEAT1/Men epsilon/beta, and the Drosophila Behavior Human Splicing family of proteins, which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells, but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited, or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene, which is also located on chromosome 13, has been identified. [provided by
组织表达
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
细胞定位
Nucleus, nucleolus. Nucleus matrix . Cytoplasm . Nucleus speckle. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in an RNA-dependent manner. May cycle between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
功能
function:Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Translated as Lys.,similarity:Belongs to the PSPC family.,similarity:Contains 2 RRM (RNA recognition motif) domains.,subcellular location:In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.,subunit:Forms heterodimers with NONO. Found in a RNP complex with CAT2 transcribed nuclear RNA (CTN-RNA). Interaction with NONO is required for its targeting to paraspeckles and perinucleolar caps. Interacts with SFPQ.,tissue specificity:Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
