RM6543

SUPT5H (16A11) Rabbit Monoclonal Antibody

hSPT5; SPT5; SPT5H; supt5h; Tat CT1;

Affinity-chromatography

常规抗体

SUPT5H

SPT5, SPT5H

WB

Human,Mouse,Rat

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

6829

O00267

A synthetic peptide of human SPT5

WB: 1:1000 IHC: 1:50 ICC/IF: 1:50

150kDa

121kDa

Rabbit IgG

SUPT5H Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Ubiquitously expressed.

Nucleus.

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

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