产品名称
Gli3 (10W4) Rabbit Monoclonal Antibody
别名
ACLS; GCPS; GLI3; GLI3FL; PAPA; PAPA1; PAPB; PHS; PPDIV;
纯度
Affinity-chromatography
存储缓冲液
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Human Swissprot No.
P10071
免疫原
Recombinant protein of human Gli3
稀释度
WB: 1:1000 IHC: 1:100 ICC/IF: 1:100
注意事项
Gli3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
组织表达
Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium
细胞定位
Nucleus. Cytoplasm. Cell projection, cilium. Note=GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus. Ciliary accumulation requires the presence of KIF7 and SMO. Translocation to the nucleus is promoted by interaction with ZIC1
功能
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI- consensus sequence 5'-GGGTGGTC-3'.
