产品名称
ULA1 Rabbit Polyclonal Antibody
蛋白名称
NEDD8-activating enzyme E1 regulatory subunit (Amyloid beta precursor protein-binding protein 1, 59 kDa) (APP-BP1) (Amyloid protein-binding protein 1) (Proto-oncogene protein 1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q13564
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q13564/entry
Mouse Swissprot No.
Q8VBW6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8VBW6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q9Z1A5
免疫原
Synthesized peptide derived from human protein . at AA range: 390-470
特异性
ULA1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
组织表达
Ubiquitous in fetal tissues. Expressed throughout the adult brain.
细胞定位
Cell membrane . Colocalizes with APP in lipid rafts.
信号通路
Alzheimer's disease;
功能
caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,enzyme regulation:Binding of TP53BP2 to the regulatory subunit NAE1 decreases neddylation activity.,function:Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.,miscellaneous:NAE1 and UBA3 correspond to the N-terminal and the C-terminal part of yeast UBA3. In yeast the two subunits form a single polypeptide chain.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.,subcellular location:Colocalizes with APP in lipid rafts.,subunit:Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2.,tissue specificity:Ubiquitous in fetal tissues. Expressed throughout the adult brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
