产品名称
NOXO1 Rabbit Polyclonal Antibody
基因名称
NOXO1 P41NOX SH3PXD5
蛋白名称
NADPH oxidase organizer 1 (NADPH oxidase regulatory protein) (Nox organizer 1) (Nox-organizing protein 1) (SH3 and PX domain-containing protein 5)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q8NFA2
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8NFA2/entry
Mouse Swissprot No.
Q8VCM2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8VCM2
免疫原
Synthesized peptide derived from human protein . at AA range: 160-240
特异性
NOXO1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes an NADPH oxidase (NOX) organizer, which positively regulates NOX1 and NOX3. The protein contains a PX domain and two SH3 domains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012],
组织表达
Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.
细胞定位
[Isoform 3]: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Isoform 3 associates with the plasma membrane in a lipid-dependent manner (PubMed:12716910).
功能
developmental stage:Isoform 3 is expressed in fetal liver.,domain:The PX domain mediates lipid-binding, localization to the plasma membrane and is required for NOX1 activation.,domain:The SH3 domains mediate interaction with CYBA/p22phox. Also mediates intramolecular interaction with the proline-rich region.,function:Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 SH3 domains.,subcellular location:Isoform 3 associates with the plasma membrane in a lipid-dependent manner.,subunit:Interacts with NOX1, NOXA1, CYBA/p22phox and NCF2/p67phox.,tissue specificity:Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
