产品名称
Calsequestrin 1 (5Q2) Rabbit Monoclonal Antibody
别名
Calmitin; Calmitine; Calsequestrin; Calsequestrin-1; CASQ; Casq1; PDIB1;
纯度
Affinity-chromatography
存储缓冲液
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Human Swissprot No.
P31415
稀释度
WB 1:1000~1:5000 IHC 1:50~1:200 FC 1:100
注意事项
Calsequestrin 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
组织表达
Expressed in myoblasts (at protein level).
细胞定位
Endoplasmic reticulum Sarcoplasmic reticulum. Sarcoplasmic reticulum lumen {ECO:0000250|UniProtKB:P07221}. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side {ECO:0000250|UniProtKB:P07221}. Mitochondrion matrix {ECO:0000250|UniProtKB:O09165}. Note=This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Preferentially forms linear and round aggregates in the endoplasmic reticulum (ER) of resting cells (PubMed:28895244). In a minority of cells, homogeneously detected in the ER lumen (PubMed:28895244). Colocalizes with STIM1 at endoplasmic reticulum in response to a depletion of intracellular calcium (PubMed:27185316). {ECO:0000250|UniProtKB:P07221, ECO:0000269|PubMed:27185316, ECO:0000269|PubMed:28895244}
功能
Calsequestrin is a high-capacity, moderate affinity, calcium- binding protein and thus acts as an internal calcium store in muscle (PubMed:
28895244). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (PubMed:
28895244). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (PubMed:
27185316).
