产品名称
FEN1 (9F12) Rabbit Monoclonal Antibody
别名
DNase IV; FEN1; Flap endonuclease 1; Flap structure specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2;
纯度
Affinity-chromatography
基因名称
FEN1 {ECO:0000255|HAMAP-Rule:MF_03140}
存储缓冲液
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Human Swissprot No.
P39748
免疫原
A synthetic peptide of human FEN1
稀释度
WB: 1:1000 IHC: 1:50-1:200
注意事项
FEN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
细胞定位
[Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage
功能
Structure-specific nuclease with 5'-flap endonuclease and 5'- 3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site- terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
