产品名称
CRISPR-Cas9 SA (19L13) Rabbit Monoclonal Antibody
别名
Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9;
纯度
Affinity-chromatography
基因名称
cas9 {ECO:0000255|HAMAP-Rule:MF_01480}
反应种属
Staphylococcus aureus
存储缓冲液
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Human Swissprot No.
J7RUA5
稀释度
WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30 FC 1:50
注意事项
CRISPR-Cas9 SA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
功能
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans- encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.
