RM5201

PABPN1 (9U19) Rabbit Monoclonal Antibody

Nuclear poly(A)-binding protein 1; PAB2; PABII; PABP2; PABPII; Pabpn1; poly(A) binding protein nuclear 1;

Affinity-chromatography

常规抗体

PABPN1

PAB2, PABP2

WB

Human,Mouse

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

8106

Q86U42

A synthetic peptide of human PABPN1

WB: 1:1000-1:5000 IHC: 1:20-1:100 ICC/IF: 1:20-1:50 FC: 1:20 IP: 1:20

50kDa

33kDa

Rabbit IgG

PABPN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Ubiquitous.

Nucleus. Cytoplasm. Nucleus speckle Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus (PubMed:10688363). Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP- sensitive import mechanism (By similarity). Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Colocalizes with SKIP and poly(A) RNA in nuclear speckles (By similarity). Intranuclear filamentous inclusions or 'aggregates' are detected in the myocytes of patients; these inclusions contain PABPN1, ubiquitin, subunits of the proteasome and poly(A) RNA {ECO:0000250|UniProtKB:Q28165, ECO:0000269|PubMed:10688363, ECO:0000269|PubMed:11001936, ECO:0000269|PubMed:11371506, ECO:0000269|PubMed:14663186, ECO:0000269|PubMed:17289661, ECO:0000269|PubMed:27209344}

Involved in the 3'-end formation of mRNA precursors (pre- mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle- specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).

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