产品名称
NEDD8 (5F5) Rabbit Monoclonal Antibody
纯度
Affinity-chromatography
基因名称
NEDD8 {ECO:0000303|PubMed:9694792, ECO:0000312|HGNC:HGNC:7732}
存储缓冲液
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Human Swissprot No.
Q15843
免疫原
A synthetic peptide of human NEDD8
稀释度
WB: 1:2000 IHC: 1:50 ICC/IF: 1:50 FC: 1:50 IP: 1:20
注意事项
NEDD8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
组织表达
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes
细胞定位
Nucleus. Note=Mainly nuclear.
功能
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:
9694792, PubMed:
10318914, PubMed:
10597293, PubMed:
11953428, PubMed:
15242646, PubMed:
14690597). Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed:
9694792, PubMed:
10318914, PubMed:
10597293, PubMed:
11953428, PubMed:
20688984). Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed:
15242646). Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed:
14690597).
