RM4698

TOP1 (15F17) Rabbit Monoclonal Antibody

DNA topoisomerase 1; DNA topoisomerase I; TOP1; TOPI; topoisomerase I; type I DNA

Affinity-chromatography

常规抗体

TOP1

WB

Human,Mouse

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

7150

P11387

A synthetic peptide of human Topoisomerase I

WB: 1:2000-1:10000 IHC: 1:20-1:100 ICC/IF: 1:20-1:50 FC: 1:20

91kDa

91kDa

Rabbit IgG

TOP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Endothelial cells..

Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)- enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

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