产品名称
GARS (9R18) Rabbit Monoclonal Antibody
别名
HMN5; CMT2D; DSMAV; GlyRS; SMAD1;
纯度
Affinity-chromatography
存储缓冲液
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Human Swissprot No.
P41250
稀释度
WB 1:1000~1:2000 IHC 1:50~1:200
注意事项
GARS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
组织表达
Widely expressed, including in brain and spinal cord. [Isoform 1]: Expressed in brain, spinal cord, muscle, heart, spleen and liver.
细胞定位
Cytoplasm. Cell projection, axon. Secreted {ECO:0000250|UniProtKB:Q9CZD3}. Secreted, extracellular exosome {ECO:0000250|UniProtKB:Q9CZD3}. Note=In transfected COS7 cells, not detected in mitochondria, nor in Golgi apparatus (PubMed:17035524) Secreted by motor neuron, possibly through the exosome pathway (By similarity). {ECO:0000250|UniProtKB:Q9CZD3, ECO:0000269|PubMed:17035524} [Isoform 2]: Cytoplasm. Cell projection, axon
功能
Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed:
17544401, PubMed:
28675565, PubMed:
24898252). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed:
19710017).
