产品名称
ATG7 (19V13) Rabbit Monoclonal Antibody
别名
ATG7; APG7-LIKE; APG7L; DKFZp434N0735; GSA7;
纯度
Affinity-chromatography
存储缓冲液
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Human Swissprot No.
O95352
免疫原
A synthetic peptide of human ATG7
稀释度
WB: 1:2000-1:10000 IHC: 1:100 ICC/IF: 1:20-1:100 FC: 1:20 IP: 1:20
注意事项
ATG7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
组织表达
Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
细胞定位
Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme
功能
E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).
