RM4606

Chk2 (11S16) Rabbit Monoclonal Antibody

CHEK2; CHK2; Cds1; Chk2; EC 2.7.11.1; RAD53; kinase Chk2;

Affinity-chromatography

常规抗体

CHEK2

CDS1, CHK2, RAD53

WB

Human

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

11200

O96017

Recombinant protein of human Chk2

WB: 1:5000-1:10000 IHC: 1:20-1:100 ICC/IF: 1:50 FC: 1:20 IP: 1:20-1:50

61kDa

61kDa

Rabbit IgG

Chk2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues

[Isoform 2]: Nucleus. Note=Isoform 10 is present throughout the cell [Isoform 7]: Nucleus. [Isoform 12]: Nucleus.

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK- cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).

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