产品名称
STK3 (13E13) Rabbit Monoclonal Antibody
别名
STK3; Mess1; MST-2; MST2; Serine/threonine kinase 3; KRS1; STE20-like kinase MST2;
纯度
Affinity-chromatography
存储缓冲液
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Human Swissprot No.
Q13188
免疫原
A synthetic peptide of human STK3
稀释度
WB: 1:5000 IHC: 1:20 ICC/IF: 1:20 IP: 1:20
注意事项
STK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
组织表达
Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
细胞定位
Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between nucleus and cytoplasm (PubMed:19525978, PubMed:11278283) Phosphorylation at Thr-117 leads to inhibition of nuclear translocation (PubMed:19525978).
功能
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.
