产品名称
MLH1 (18Q4) Rabbit Monoclonal Antibody
别名
DNA mismatch repair protein Mlh1; MutL protein homolog 1; COCA2;
纯度
Affinity-chromatography
存储缓冲液
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.
Human Swissprot No.
P40692
免疫原
A synthetic peptide of human MLH1
稀释度
WB: 1:2000 IHC: 1:50 ICC/IF: 1:50 FC: 1:20 IP: 1:20
注意事项
MLH1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
组织表达
Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart
细胞定位
Nucleus. Chromosome Note=Recruited to chromatin in a MCM9-dependent manner
功能
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
