产品名称
MCF2L Rabbit Polyclonal Antibody
蛋白名称
Guanine nucleotide exchange factor DBS (DBL's big sister) (MCF2-transforming sequence-like protein)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
O15068
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O15068/entry
Mouse Swissprot No.
Q64096
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q64096
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q63406
免疫原
Synthesized peptide derived from part region of human protein
特异性
MCF2L Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],
组织表达
Amygdala,Brain,Muscle,PCR rescued clones,
细胞定位
[Isoform 5]: Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .; [Isoform 3]: Cytoplasm . Endomembrane system . Interaction with membranes enriched in phosphoinositides is mediated by the CRAL-TRIO domain. .; Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .
功能
domain:The CRAL-TRIO domain is involved in interaction with inositol phospholipids.,domain:The DH domain is involved in interaction with CCPG1.,function:Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.,similarity:Belongs to the MCF2 family.,similarity:Contains 1 CRAL-TRIO domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 1 spectrin repeat.,subunit:Interacts with CCPG1, which results in specific inhibition of its exchange activity toward RHOA (By similarity). Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
