产品名称
TPPP Rabbit Polyclonal Antibody
别名
Tubulin polymerization-promoting protein (TPPP) (25 kDa brain-specific protein) (TPPP/p25) (p24) (p25-alpha)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11076
Human Swissprot No.
O94811
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O94811/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/72948
Mouse Swissprot No.
Q7TQD2
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q7TQD2
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/0
特异性
This antibody detects endogenous levels of TPPP at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
细胞定位
Golgi outpost . Cytoplasm, cytoskeleton, microtubule organizing center . Cytoplasm, cytoskeleton . Nucleus . Cytoplasm, cytoskeleton, spindle . Specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, which shapes dendrite morphology by functioning as sites of acentrosomal microtubule nucleation (By similarity). Mainly localizes to the cytoskeleton (PubMed:18028908). Also found in the nucleus; however, nuclear localization is unclear and requires additional evidences (PubMed:18028908). Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies (PubMed:15590652, PubMed:17027006). During mitosis, colocalizes with LIMK2 at the mitotic spindle (PubMed:22328514). .
功能
function:Promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.,PTM:Phosphorylated by TPK II. Poor substrate for GSK3.,similarity:Belongs to the TPPP family.,subunit:May form dimers. Binds tubulin; binding is inhibited by GTP. Interacts with GSK3.,tissue specificity:Widely expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
