产品名称
IRE1 Phospho-thr973 Rabbit Polyclonal Antibody
别名
Serine/threonine-protein kinase/endoribonuclease IRE1 (Endoplasmic reticulum-to-nucleus signaling 1) (Inositol-requiring protein 1) (hIRE1p) (Ire1-alpha) (IRE1a) [Includes: Serine/threonine-protein kinase (EC 2.7.11.1); Endoribonuclease (EC 3.1.26.-)]
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2081
Human Swissprot No.
O75460
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O75460/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/78943
Mouse Swissprot No.
Q9EQY0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9EQY0
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/0
特异性
This antibody detects endogenous levels of IRE1 thr973 at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Ubiquitously expressed. High levels observed in pancreatic tissue.
细胞定位
Endoplasmic reticulum membrane ; Single-pass type I membrane protein .
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.,function:Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 KEN domain.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1.,tissue specificity:Ubiquitously expressed. High levels observed in pancreatic tissue.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
